Preall Laboratory / CSHL Single Cell Biology Shared Resource

The Preall Laboratory shares space and equipment with the laboratory of Dick McCombie and the Next Generation Sequencing Shared Resource at the CSHL Woodbury Genome Center. The Preall Laboratory owns or shares access to all necessary common laboratory equipment such as microcentrifuges, gel running equipment, multi-channel pipettes, incubators, tissue culture hoods, fume hoods, microscopes, freezers and cryostorage.

 

Single Cell/Nucleus Sequencing:

The following equipment is critical to standard operations in the Single Cell Biology Shared Resource running single cell/nucleus RNAseq, nuc ATACseq, multiomics, and other bespoke genomics assays:

10X Genomics Chromium X

10X Genomics Chromium Controller

Sony SH800 flow cytometer and cell sorter

Countess II FL automated fluorescent cell counter

EVOS FL Fluorescence Microscope

 

Spatial Biology:

The proposed spatial transcriptomics experiments will make heavy use of the following equipment located at the Woodbury Genome Center:

10X Genomics Visium CytAssist

10X Genomics Xenium In Situ Analyzer

 

CSHL Next Generation Sequencing Shared Resource Equipment:

The following instrumentation is available at the Woodbury Genome Center with shared access by the Preall and McCombie laboratories.  This includes all necessary equipment for standard NGS library prep, QC, and sequencing of libraries proposed for this Atlas:

Tissue culture hoods and incubators

Refrigerated swinging bucket centrifuges (several)

MJ Research / Biorad tetrad thermal cyclers (6)

Qubit fluorimeter

BioRad Gel Doc EZ Gel Imager

Applied Biosystems 7900HT Fast Real Time PCR system

Nanodrop ND1000 spectrophotometer

Agilent 2100 Bioanalyzer

Agilent TapeStation 4100

SageElf DNA size selection system

Agilent Femto Pulse high molecular weight DNA fragment analyzer

Illumina NextSeq 2000, 500, and MiSeq sequencers

Pacific Biosciences Sequel sequencers

Oxford Nanopore PromethION, GridIon, and MinIon sequencers

 

 

CSHL Animal and Tissue Imaging Shared Resource: A fully equipped histology shared resource is co-located at the Woodbury Genome Center, adjacent to the Preall lab and managed by Dr. Qing Gao. This facility will be critical for staining and imagine Visium and Xenium slides, and houses the following key equipment:

 

Thermo Microtome: HM355S, Finesse, FinesseME and ME+

Thermo Fisher Scientific Tissue Processor Shandon Excelsior ES

Thermo HistoStar Embedding station

Thermo Cryostat # NX50

Leica Cryostat CM 3050S

Leica Multi Stainer ST5020

Leica Glass Coverslipper CV5030

Olympus VS200 slide scanner

Roche Discovery Ultra Automated IHC/ISH platform

Leica Microdissection System LMD7000