CSHL Single Cell Biology Core
Spatial Biology
@
CSHL
Services
We are proud to offer the following spatial biology services:
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In Situ Profiling
10X Genomics Xenium
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Off-the-shelf panels of 350-00 target genes
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Customizable add-on panels of 50-100 genes
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Co-stain with H&E and immunofluorescence
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1cm x 2cm imaging area per slide
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mouse and human panels
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Spatial Transcriptomics
10X Genomics Visium FFPE
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Whole-transcriptome analysis
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6mm x 6mm tissue sections, 4 per slide
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100um center-to-center spot resolution
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Co-stain with H&E or immunofluorescence
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mouse and human panels
10X Genomics Visium CytAssist
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Transfer existing, imaged slide sections to Visium
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No need to cutting & sectioning
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Getting Started With Spatial
Pair the right sample with the right assay:
​Both Xenium and Visium assays require tissue to have high quality RNA but depending on the assay FFPE blocks, Fresh Frozen blocks, or pre cut slides can be used as input​
How to Submit Samples for Visium CytAssist:
Stained slides:
​You send us prestained slides with a hardset coverslip and select your ROI.​
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We check RNA quality and if everything looks good we proceed with the Visium workflow and provide you with raw sequencing data, images and the preliminary Space Ranger analysis of your visium HD or SD data.
FFPE Blocks:
​You send us FFPE blocks ​
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We section, check RNA quality, stain and image them
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You select ROIs
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We proceed with the Visium workflow and provide you with raw sequencing data, images and the preliminary Space Ranger analysis of your visium HD or SD data.
